Code for analysis of lysosome distance from nucleus

Cells were cultured in Ibidi 4-well µ-slides for three days. Prior to imaging, the medium was replaced with HEPES-buffered D5030 medium titrated to pH 7.4. Lysotracker Green DND 26 and Hoechst33342 were added to the medium at concentration of 100 nM and 10 µg/mL, respectively. After 10 min of incubation, Lysotracker fluorescence was recorded at 488 nm excitation, emission >510 nm using Zeiss LSM 700 confocal microscope.