Spathaspora passalidarum NRRL Y-27907 Transcriptome or Gene expression

Xylose fermentation is a valuable trait for the second-generation ethanol industry. Xylose-fermenting yeasts are microorganisms still poorly exploited but with great potential to be used as a source of genes or as cell factories in fermentative processes. Overall, this group of yeasts presents low ethanol tolerance, which is one of the reasons hindering their industrial applications. An RNA-seq analysis was performed to investigate S. passalidarum transcriptional changes in response to two hours of 4% (v/v) ethanol stress aiming to shed light on the mechanisms underlying the low ethanol tolerance profile of this yeast and search for clues to improve this phenotype

木糖发酵是第二代乙醇工业中一项宝贵的特性。木糖发酵酵母作为一种微生物，尽管尚未得到充分开发，却具有巨大的潜力，可被用作基因来源或在发酵过程中的细胞工厂。总体而言，这一类酵母对乙醇的耐受性较低，这是阻碍其工业应用的原因之一。为了探究S. passalidarum在受到4%（体积比）乙醇胁迫两小时后的转录组变化，进而阐明该酵母低乙醇耐受性机制，并寻找改善这一表型的线索，进行了RNA测序分析。