Expression data of HSFA6b-overexpression and HSFA6b dominant-negative mutant lines of Arabidopsis after NaCl or Heat shock treatment

In order to study the improtance of HSFA6b in salt and heat stresses of arabidopsis, we generated the HSFA6b-overexpression (HSFA6b-OE) and dominant-negative (HSFA6b-RD) mutant lines. We used microarray to investigate how genes regulated by HSFA6b after NaCl or heat shock treatment. Seven-day-old seedlings of wild type (Col-0), HSFA6b-overexpression (HSFA6b-OE) and HSFA6b dominant-negative (HSFA6b-RD) mutant lines grown at 22℃ on 0.5X MS plates containing 1% sucrose were treated with 150mM NaCl for 6hr or 37℃ heat shock for 1hr, with 22℃ treatment as a control. Subsequently, samples were collected for RNA extraction by use of the RNeasy Kit (Qiagen). The results were representative of two independent biological replicates for analysis.