Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer.

<strong>qRT-PCR </strong> Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit. Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii. The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells for analysis.

<strong>实时荧光定量逆转录聚合酶链反应（qRT-PCR）</strong>：采用TRIzol试剂提取细胞总RNA，取500 ng总RNA通过PrimeScript试剂盒逆转录为cDNA。以肌动蛋白（Actin）引物作为内参。采用Lightcycler 480ii荧光定量PCR仪完成qRT-PCR检测。本研究所用的qRT-PCR引物序列如下：Cacybp：正向引物：5-CTCCCATTACAACGGGCTATAC-3，反向引物：5-GAACTGCCTTCCACAGAGATG-3；hactin：正向引物：5-GGCATCGTCACCAACTGGGAC-3，反向引物：5-CGATTTCCCGCTCGGCCGTGG-3。获取HK-2、O-786、SV-HUC-1、T24细胞中肌动蛋白及CACYBP基因的Cq值数据用于后续分析。