Microbial community dynamics in a simplified recirculating aquaculture system co-culturing Hippocampus abdominalis and Penaeus japonicus with integrated biofiltration

This project characterizes bacterial microbiota in a commercial-scale simplified recirculating aquaculture system (RAS) for sustainable seahorse (Hippocampus abdominalis) farming. The RAS integrates zero-water-exchange operation and biofiltration via co-culture with kuruma shrimp (Penaeus japonicus). We compared microbial communities across three compartments:FTS-CT: Flow-through system culture tank water (control)RAS-CT: Recirculating system culture tank waterRAS-BT: Biofilm reactor tank carrier-attached biomass