RNA-seq profiling of 368T1 murine KP NSCLC cells deleted for AMPK with AMPKa1 add-back subjected to high glucose conditions, Replicate 1

This dataset was generated for the paper, "Genetic analysis reveals AMPK is required to support tumor growth in murine Kras-dependent lung cancer models." Murine Kras mutant, p53 null (KP) 368T1 NSCLC cells were deleted for AMPK using the CRISPR/Cas9 system and subsequently stably infected with control vector ("KO") or AMPKalpha1 cDNA ("A1") add-back. These cells were subjected to high glucose (25mM, "HG") conditions for 12 or 18 hours, and profiled by RNA-sequencing. Replicate 1 High Glucose (HG) and No Glucose (NG) conditions were generated simultaneously and analyzed together. Analysis of a single dataset was performed using Replicate 1 ("R1"), and both replicates ("R1" and "R2") were analyzed together as indicated.

本数据集旨在支持论文《遗传分析揭示AMPK对于支持小鼠Kras依赖性肺癌模型肿瘤生长的必要性》的研究。采用CRISPR/Cas9系统对小鼠Kras突变型、p53缺失型（KP）368T1非小细胞肺癌细胞进行AMPK基因的敲除，随后分别稳定转染控制载体（“KO”）或AMPKalpha1 cDNA（“A1”）。这些细胞在12小时或18小时的高葡萄糖（25mM，“HG”）条件下培养，并通过RNA测序进行表征。同时生成并分析了高葡萄糖（HG）和无葡萄糖（NG）条件下的重复1（“R1”），并按指示对单个数据集进行重复1（“R1”）的分析，同时将两个重复（“R1”和“R2”）的数据集进行综合分析。