Annotation file for de novo assembled transcriptome of hybrids between Epidendrum fulgens and E. puniceoluteum (Orchidaceae)

We used Blast2GO to perform a functional annotation of the de novo assembled transcriptome of hybrids between Epidendrum fulgens and E. puniceoluteum. To do so, we filtered for contigs longer than 300 bp and then aligned the contigs against the NCBI non-redundant NR protein database with an e-value cut-off of 10e−3 using the CloudBlast service. Simultaneously, we used the InterProScan tool to retrieve protein domains and motif information. Lastly, we processed all matching transcripts and assigned gene ontology (GO) terms following the Blast2GO annotation workflow.