scRNAseq of innate lymphoid cells generated in vitro from umbilical cord blood derived CD34+ hematopoietic progenitors.

Innate lymphoid cells (ILCs) were generated in vitro from CD34+ hematopoietic progenitors derived from umbilical cord blood, and RNA sequencing was performed on CD45+ LineageLD- cells to assess transcriptional identities of lineages generated ILCs were generated through in vitro culture from CD34+ HPCs as described in the manuscript, from 2 independent donors. Cells from conditions OP9+IL-15, DL1+IL-15 and DL1 were FACS-sorted as CD45p cells which were Lineage (CD14, CD123, FcERa, Viability dye) negative, combined with the following CITE-Seq 34 anitbodies: CD117, CD304(Neuropilin-1), CD254 (RANKL), CD336 (NKp44), CD196(CCR6), CD34, CD7, CD127(IL-7Rα), CD1a, CD5, CD3(SK7), CD4, CD8a, TCRα/β, TCRγ/δ, CD183(CXCR3), CD94, NKp80, CD335(NKp46), CD294(CRTH2), CD278(ICOS), HLA-DR, integrinβ7, CD49d (a4), CD19, CD161, CD2, KLRG1, CD56, CD16, Siglec-7, KIR2DL1, KIR2DL2, KIR3DL1. Cells were individually hashtaged per donor and per condition.