Brightfield images: p161, p163

This repository constains all the brightfield plate scans plate p161, p163 for the project: A quantitative analysis of the interplay of environment, neighborhood, and cell state in 3D spheroids Vito RT Zanotelli Matthias Leutenegger Xiao‐Kang Lun Fanny Georgi Natalie de Souza Bernd Bodenmiller Mol Syst Biol. (2020) 16: e9798 https://doi.org/10.15252/msb.20209798 Please cite this article if you re-use any of the data or code. Each plate acquisition follows the naming scheme: '{date}-{operator}-Spheroid-{plate}[rev]-{dps}-ac{acnr}_Plate_{acquisitionid} eg. 20180203-Vito-Spheroid-p173-2dps-ac1_Plate_13146 20180204-Vito-Spheroid-p173rev-3dps-ac1_Plate_1315 With: date: acquisition date operator: microscope operator plate: plate number rev: optional, if there indicates that the plate has been rotated 180 degree. E.g. well A01 will be well H12. dps: days post seeding ac: manual acquisition number (e.g. if acquisition needed to be repeated) acquisitionid: machine provided unique acquisition id Each folder contains: HTD files which contains acquisition metadata in text form. TimePoint_1: a folder containing all the acquisitions in .tiff form with the suffix _{wellname}_w{channel} In this acquisition channels 1-3 correspond to 3 brightfield acquisitions at different z positions. Acquisition channel 4 corresponds to an acquisition of the GFP channel. An ImageXpress Micro XL Widefield High Content Imaging microscope (Molecular Devices, 4x objective, NA 0.20) was used for the acquisition.