Long EdU pulse to study <i>Nematostella</i> cell proliferation after feeding (Fig. 4L)

Per sample a group of 10 juvenile polyps were labeled for 24 to 48h with EdU (300uM, 2%DMSO) in several intervals after feeding and then dissociated using ACME. Cells were stained with 1μg/ml FxCycle violet DNA dye (Invitrogen) and EdU was labelled with Alexa488 using a Click-it reaction. Flow cytometry was performed a BD LSRFortessa (BD Life Sciences) and the EdU positive cells in samples were determined based on Alexa488 fluorescence in comparison with the DMSO controls using FlowJoV10.8 (BD Life Sciences)..wsp files contain the samples, gating strategy and cell populations used for the analysis in FlowJo.fcs files represent the individual flow cytometry output files that were analysed in the .wsp file<br>每例样本取10枚幼年息肉，于喂食后多个时间间隔内使用终浓度300μM、含2%二甲基亚砜（DMSO）的5-乙炔基-2'-脱氧尿苷（EdU）标记24至48小时，随后采用ACME法进行细胞解离。使用1μg/ml的FxCycle Violet DNA染料（Invitrogen）对细胞进行染色，并通过Click-it反应将EdU与Alexa488荧光基团偶联标记。采用BD LSRFortessa流式细胞仪（BD生命科学公司）开展流式细胞检测，借助FlowJoV10.8软件（BD生命科学公司），以DMSO对照组为参照，通过Alexa488荧光信号强度判定样本中的EdU阳性细胞比例。.wsp格式文件包含了FlowJo软件分析时所用的样本信息、门控策略及细胞群划分结果；.fcs格式文件则为对应.wsp文件中所分析的单一流式细胞检测原始输出文件。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于使用长时EdU脉冲技术研究喂食后Nematostella vectensis（一种海葵）的细胞增殖过程，具体关联到研究中的图4L。数据集包含流式细胞术分析的原始文件（.fcs和.wsp格式），实验方法涉及对幼年息肉进行EdU标记、细胞解离和荧光染色，以量化增殖细胞比例。数据集发布于2024年，由Kathrin Garschall贡献，适用于动物发育生物学和细胞生理学领域的研究。