The primer and probe sequences used for detecting specific miRNAs and other small RNAs.

*The lowercase letters indicate the reverse complementary nucleotides in the primer sequence. The specificity of stem-loop RT primers to individual miRNA is conferred by a 6-nt extension at the 3′ end; this extension is a reverse complement of the last six nucleotides at the 3′ end of the miRNA. Forward primers are specific to the miRNA sequence but exclude the last six nucleotides at the 3′ end of the miRNA.