Yeast Solutions and Hyperpolarization Enable Real-Time Observation of Metabolized Substrates Even at Natural Abundance

This data corresponds to the following paper: Title: Yeast Solutions and Hyperpolarization Enable Real-Time Observation of Metabolized Substrates Even at Natural Abundance Journal: Analytical Chemistry DOI: 10.1021/acs.analchem.4c02419 Authors: Josh P. Peters, Charbel Assaf, Farhad Haj Mohamad, Eric Beitz, Sanjay Tiwari, Konrad Aden, Jan-Bernd Hövener and Andrey N. Pravdivtsev The data is organized with respect to the subfigures in figure 2 to 6. A description of acquisition parameters is provided in the "Acquisition parameters.xlsx" file for each dataset. Figure 2: - Overview of pyruvate metabolism in yeast cells using hyperpolarized and 13C labeled [1-13C]pyruvate. Hyperpolarized and thermally polarized spectra, if applicable, are included. Figure 3: - Overview of pyruvate metabolism in yeast cells using co-hyperpolarized [1-13C] and [2-13C]pyruvate at an n.a. of 13C. Hyperpolarized and thermally polarized spectra, if applicable, are included. Figure 4: - Overview of fumarate metabolism in yeast cells using hyperpolarized and 13C labeled [1,4-13C2]fumarate Hyperpolarized and thermally polarized spectra, if applicable, are included. Figure 5: - The fitted conversion exchange rate constants as a function of the yeast concentration. Hyperpolarized and thermally polarized spectra, if applicable, are included. Figure 6: - Metabolic data from pyruvate metabolism in yeast depending on the position of the yeast in the NMR tube. We Hyperpolarized and thermally polarized spectra, if applicable, are included.