Real-time polymerase chain reaction primers and dual labeled hydrolysis probes developed in this study for detection of <i>Colletotrichum cereale</i> subspecific groups.
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aPrimer/probe sets reside within Apn2 locus. bFAM = 6-carboxy-fluorescein fluorescent reporter dye (IDT, Coralville, IA). cZEN = internal quencher to enhance specificity (IDT, Coralville, IA). dIBFQ = Iowa Black Fluorescent Quencher (IDT, Coralville, IA).
创建时间:
2014-05-19




