Genome-wide study of the role of Tox4 on gene expression in adult Drosophila ovaries

RNA Seq analysis of total RNA from adult ovaries to determine differential gene expression between the following genotypes: w[1118] control strain, homozygous tox4 null allele, with or without GFP-tox4 transgenic rescue, homozygous PNUTS null allele with transgenic constructs disrupting PP1-binding or tox4-binding, each in triplicate. Read depth was approx. 45-55M reads/replicate.