Proteomic profiling of UV damage repair patches

DNA damage compromises not only genome stability but also the integrity of the chromatin template, which plays a central role in controlling cell identity. Our understanding of chromatin repair mechanisms is very incomplete. To bridge this knowledge gap, here we devised a novel proteomic strategy to characterize dynamic changes in the chromatin landscape during the repair of UV-induced DNA lesions in human cells, in a quantitative, unbiased and time-resolved manner. Based on the established IPOND methodology (Isolation of Proteins On Nascent DNA), we called this method IPOND-R for Isolation of Proteins On Nascent DNA at Repair sites.