Examining the role of ATRX-RNA interactions in ATRX and PRC2 localization and gene expression.

We disrupted the RNA binding region in endogenous ATRX and tested localization of ATRX, EZH2 and H3K27me3 by CUT&RUN. We show that ATRX-RNA binding is important to regulate ATRX and PRC2 localization to specific genes. We performed CUT&RUN for ATRX, EZH2 and H3K27me3 in WT and ATRX△RBR (ATRX with RNA binding region deleted) using the protocol described in Skene et al, Nature protocols, 2018). RNA Seq was performed in WT and ATRX△RBR.