single cell RNA-seq in transplanted bone marrow cells from SYNCRIP conditional mouse model

SYNCRIP depletion results in HSCs losing their self renewal abilities. Single cell sequencing was conducted in SYNCRIP WT and KO bone marrow LK (lineage -, cKit+) cells to decipher the effect of Syncrip deletion on cellular identities along the hematopoietic hierarchy, and to gain an in-depth assessment of the transcriptomic changes in different cell types upon SYNCRIP loss. SYNCRIP flox/flox conditional knockout mice were developed for this study. Bone marrow cells from these conditional knockout mice were harvested and transplanted into CD45.1 congenic mice, 6 weeks post transplant mice were intraperitoneal injected with polyinosinic:polycytidylic acid (pIpC). 3 weeks following injections, the transplanted LK (lineage-, cKit+) cells were FACS sorted and used for single cell RNA-seq. 10x Genomics