Transcriptomic responses to oil, a pathogen, or both in spleen and liver tissues from juvenile red drum (Sciaenops ocellatus)

The objective of this study was to determine how transcriptomic responses in the spleen and liver are altered in juvenile Sciaenops ocellatus exposed to oil, a known fish pathogen (Vibrio anguillarum), or both. S. ocellatus juveniles were exposed to oil, a pathogen (Vibrio anguillarum) or both. Oil exposure was for 5 days and pathogen exposure was for 1 hour, 24 hours before takedown. Oil solutions used in exposures were derived from high energy water accommodated fractions (HEWAF) prepared using Macondo well oil from the Deepwater Horizon oil spill following established procedures. At the end of the experiments, livers and spleen were taken from organisms, RNA was extracted and sequenced.