SSH libraries of the olive tree (Olea europaea L.) reproductive tissues

The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process which determines the yield. The use of subtractive SSH from pollen and pistil libraries constructed at specific stages may help to reveal the specific transcripts involved may help to elucidate the events and signalling processes which happen during the courtship, pollen grain germination, and fertilization in olive. Such libraries have also been created by subtracting vegetative mRNAs (from leaves), in order to identify reproductive sequences only. Six SSH subtractive libraries have been generated by using the following combinations of tester(T)/driver(D) tissues: - T:pollen D:pistil -> Po_(P) -> pollen subtracted with pistil - T:pistil D:pollen -> P_(Po) -> pistil subtracted with pollen - T:pistil D:leaf -> P_(L) -> pistil subtracted with leaf - T:leaf D:pistil -> L_(P) -> leaf subtracted with pistil - T:pollen D:leaf -> Po_(L) -> pollen subtracted with leaf - T:leaf D:pollen -> L_(Po) -> leaf subtracted with pollen