遇见数据集

The amounts of the LO-B<sub>n</sub> fusion proteins and the HPLC-purified recombinant Buf IIIb-L monomers obtained from 1×10<sup>11</sup> cells of <i>E. coli</i>.

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NIAID Data Ecosystem2026-03-07 收录
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aTotal protein concentration was determined by BCA protein assay using bovine serum albumin as a standard. bThe amounts of the total expressed LO-Bn fusion proteins and the surface displayed LO-Bn fusion proteins were determined by quantifying the protein bands of SDS-polyacrylamide gels by densitometry at 600 nm as described in Materials and Methods. cThe amounts of the HPLC-purified recombinant Buf IIIb-L monomers were calculated using the calibration curve constructed by plotting the average peak area obtained by HPLC versus the concentration of the synthetic Buf IIIb-L peptide.

创建时间:
2013-03-14
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