scRNA-seq Analysis of Lung Cells in Influenza A Virus (IAV) Infected Aged Mice

Seasonal influenza causes significant morbidity and mortality worldwide, with 70-90% of these deaths occurring in individuals aged 65 or older. To explore the mechanisms behind this age-related vulnerability, we investigated immune responses and inflammation in the lungs of young and old mice during influenza A virus (IAV) infection. Young (12-week) and old (70-week) C57BL/6J mice were intranasally infected with IAV PR8, and immune responses were assessed at 7 days post-infection (dpi) using single-cell RNA sequencing (scRNA-seq). Overall design: scRNA-seq profiling in the lungs from old and young mice at day 7 after influenza virus infection