FACS characterization and comparison of tumor single cell suspensions derived from infected and uninfected HuH7 and PLC tumors.

AMarkers: MHCII-PE antibody detects a polymorphic determinant present on B cells, monocytes, macrophages and dendritic cells. F4/80-APC antibody recognises the F4/80 antigen, that is expressed by a majority of mature macrophages and is the best marker for this population of cells. CD19 is expressed on B cells and follicular dendritic cells. Ly6G-PE, also known as Gr-1- antigen, is expressed on mouse neutrophils, predominantly granulocytes. BSingle cell suspensions derived from infected and uninfected HuH7 and PLC tumors 7dpi (n = 4 for uninfected and n = 3 for virus-infected groups) were used for FACS characterization. Ratios greater than 1 indicate an increased accumulation of host immune cells. CGFP-positive cells of the infected tumors were presented as mean values (n = 3) +/− standard deviations in percentages.