BRAF Pyrosequencing analysis of cases with discordant results between ASLNAqPCR and Sanger sequencing.

SSEQ, Sanger sequencing; ASLNA, allele specific quantitative PCR using 3′-locked nucleic acid modified primers (ASLNAqPCR); WT, wild type; NP, not performed due to lack of additional DNA; CRC, colonic adenocarcinoma; met, metastatic; LN, lymph node; PTC papillary thyroid carcinoma. aASLNAqPCR primers designed to identify only the BRAF V600E mutation. bReal time ASLNAqPCR quantitative data. cPercentage of the tumor/non neoplastic cells ratio estimated in the area dissected for DNA extraction.