RenSeq on Sr27 carrying triticale variety Coorong and 4 susceptible EMS mutants

Seeds of triticale variety Coorong carrying stem rust resistance (R) gene Sr27 were subjected to EMS mutagenesis. Screening of M2 families with wheat stem rust pathogen Pgt21-0 inoculation identified several susceptible mutants. Genomic DNA from wildtype and 4 susceptible mutants were enriched using a bait library specific for Triticeae NB-LRRs (V2). This NLR gene capture was performed by Arbor Biosciences (Ann Arbor, USA) following the myBaits protocol. Enriched libraries were sequenced by HiSeq 2500 (Illumina) to produce 250 bp paired-end reads.