Figure 4_Nanopore seq analysis.xlsx

<strong>Figure 4: </strong>Amplicon sequencing to identify transcripts of <em>CALCR</em> expressed during treatments across the period of 24 hr. Panels A-C show the quality of reads of bar-coded samples from all three experimental repeats, respectively. The expected length of the bar-coded samples is 3.4 kb, reads with quality score below 10 were excluded and only transcripts with a minimum occurrence of 500 times were included. Panels D-F show the frequency per million of the three transcripts (Dark grey: transcript 2 CTa Receptor, Light grey: transcript 2 CTb Receptor: refer to Table 1 for details of exon and intron inclusion) expressed over the 24 hr time period. Panel D is data from partially starved cells [depleted FBS], panel E is data from cells starved of FBS [no FBS] and panel F is data from cells treated with 1 µM staurosporine. The error bars represent the variation from the mean (+/-SD) of 3 biological replicates.