Prokaryotes of Halichondria panicea exposure experiment to crude oil and dipsersant contaminated sediments

This study aimed at exposing cold-water inter-tidal sponge Halichondria panicea to crude oil and dispersant contaminated sediments. First water accommodated oil fraction (WAF), chemically enhanced water accommodated oil fraction (CEWAF) and dispersant contaminated seawater were produced and mixed with freshly collected sediments. Concentration of prokariotic organisms were assess by DAPI staining in both the contaminated seawater and sediment samples. H. panicea samples were then be exposed to the different types of contaminated sediments for 48h. Clearance rate and expression changes in sponge samples were determined during the exposure.

本研究旨在将冷水潮间带海绵 Halichondria panicea 暴露于原油和分散剂污染的沉积物中。首先制备了含油水分配率（WAF）、化学增强含油水分配率（CEWAF）及受分散剂污染的海水，并将其与新鲜采集的沉积物混合。通过在受污染的海水和沉积物样本中采用DAPI染色法对原核生物的浓度进行评估。随后，将 H. panicea 样本暴露于不同类型的污染沉积物中，持续时间为48小时。在暴露过程中，测定了海绵样本的清除率和表达变化。