Transcriptional profile of NOMO1 knockout cell lines [Affymetrix]

We report the data of whole Gene expression of three different cell lines, HT-29 (early-onset colorectal cancer, EOCRC), HCT-116 (EOCRC) and HS-5 (bone marrow) in order to analyze the transcriptional profile generated after NOMO1 (Nodal Modulator 1) gene inactivation, a candidate biomarker in EOCRC. The generation of the NOMO1 knockout cell lines was performed using CRISPR/cas9 technology. The main objective was to identify whether the loss of NOMO1 generated a differential transcriptional profile in each cell line and whether it was possible to identify genes commonly deregulated in the three NOMO1-KO cell lines. The analysis revealed a set of differentially expressed genes in common to the three cell lines as a consequence of NOMO1 inactivation. Examination of the transcriptional profile of HT-29, HCT-116 and HS-5 NOMO1 knockout cell lines.