Data from: Isolation of diverse simian arteriviruses causing hemorrhagic disease

Genetically diverse simian arteriviruses (simarteriviruses) naturally
infect geographically and phylogenetically diverse monkeys, and
cross-species transmission and emergence are of considerable concern.
Characterization of most simarteriviruses beyond sequence analysis has not
been possible because the viruses fail to propagate in the laboratory. We
attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1,
Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus
1, by inoculating an immortalized grivet cell line (known to replicate
simian hemorrhagic fever virus), primary macaque cells, macrophages
derived from macaque induced pluripotent stem cells, and mice engrafted
with macaque CD34+-enriched hematopoietic stem cells. The combined effort
resulted in successful virus isolation; however, no single approach was
successful for all 4 simarteriviruses. We describe several approaches that
might be used to isolate additional simarteriviruses for phenotypic
characterization. Our results will expedite laboratory studies of
simarteriviruses to elucidate virus-host interactions, assess zoonotic
risk, and develop medical countermeasures.