Gene expression in liver and hypothalamus from ob/ob mice treated with γ-oryzanol-encapsulated nanoparticles. Mus musculus

Brown rice-specific bioactive substance, γ-oryzanol ameliorates fuel dysmetaborism, but it is hardly absorbed form the intestine. To increase the efficiency of its absorption, we therefore challenged to encapsulate γ-oryzanol in polymer poly (DL-lactide-co-glycolide) (PLGA) nanoparticles. We assessed the metabolically-beneficial impact of PLGA-encapusulated γ-oryzanol on fuel dyshomeostasis in genetically obese-diabetic ob/ob mice. Overall design: Obese diabetic ob/ob mice were orally administered with γ-oryzanol or γ-oryzanol-encapsulated nanoparticles for 4 weeks (n=3 animals per group). Liver and hypothalamus RNA was extracted and hybridized to Affimetrix microarrays and gene expression data was analyzed.