RNA Polymerase Pausing in Human Cells

This study identifies where RNA polymerase pauses on human DNA, and determines how RNA polymerase pausing is regulated. RNA polymerase pausing is increasingly recognized as a key regulatory step in transcription that affects gene expression. We mapped the locations of RNA polymerases in human cells by the precision nuclear run-on assay (PRO-seq) and located the NELF and DSIF complexes by ChIP-seq. The data set contains technical replicates of PRO-seq from foreskin fibroblasts, NELFA and SPT5 ChIP-seq from foreskin fibroblasts, and PRO-seq from a renal proximal tubule cell line (HK-2).