Annotated Whole-Slide Images of Core Needle Prostate Biopsies

We present an expert-annotated dataset of image-guided prostate core needle biopsies (CNBs) designed to support automated analysis in the context of prostate cancer diagnosis. The dataset comprises 37 whole slide images (WSIs), corresponding to 68 hematoxylin and eosin (H&E)-stained CNBs obtained from 32 patients. A total of 15,480 manual expert annotations are included, covering a wide range of tissue types and histopathological structures commonly encountered in routine diagnostic practice. These include benign glands, prostate cancer tissue, stroma, adipose tissue, nerves, nerve ganglia, blood vessels, muscle, and inflammatory cells. Additional annotations capture relevant morphological features such as stromal retraction, normal and atypical glandular secretions, as well as tissue and digitization artifacts. All annotations were performed by two experienced uropathologists, with final labels established through consensus review. Immunohistochemical staining was used as a supporting reference when required to improve diagnostic accuracy. This dataset is intended to serve as a benchmark for developing and evaluating machine learning models for prostate biopsy analysis, and is released openly to promote reproducibility and further research in computational pathology.