Binding and regulation of transcription by yeast Ste12 variants to drive mating and invasion phenotypes

Here, we examine how six single amino acid variants in the DNA-binding domain of Ste12 – a yeast transcription factor regulating mating and invasion – alter Ste12 genome binding, motif recognition and gene expression to yield markedly different phenotypes. Using a combination of the calling card method, RNA sequencing , we find that variants with dissimilar binding and expression profiles can converge onto similar cellular behaviors. Overall design: We overexpress six variant of transcription factor Ste12 in yeast S.cervesiae and detected its genome-wide binding pattern and RNA expression pattern