YvcL-regulated genes in Enterococcus faecalis

The goal was to identify genes regulated by YvcL (EF0768) by assessing changes in the yvcL deletion mutant relative to the isogenic wild-type. Wild-type E. faecalis OG1 and the mutant lacking yvcL were cultured in Mueller-Hinton broth to exponential phase. Total RNA was purified, depleted for rRNA, and subjected to preparation of mRNA libraries. Overall design: Four biological replicates of each strain (wild-type, yvcL deletion mutant) were prepared. Cultures of the strains were grown at 37 C in Mueller-Hinton broth until exponential phase prior to collection and RNA extraction.