Supplemental Material for Ichikawa et al., 2021

<br><br>Table S1. Strains.<br><br>C. albicans strains and their genotypes are listed.<br><br> <br><br>Table S2. Primers.<br><br>Sequences of primers used in this study are indicated.<br><br> <br><br>Table S3. RNA-seq differential expression analysis.<br><br>Transcripts that respond significantly (&gt;2-fold change and p-value &lt; 0.05) to caspofungin addition in the wild type (column label “Wt+caspo/ Wt_no_caspo”) or to the cup9Δ/Δ genotype (column label “cup9+caspo/Wt+caspo”) are indicated. Log2-fold-change values are listed; the entry “Inf” indicates infinite change; the entry “0” indicates no significant change. Separate tabs are used for all RNAs (including non-coding RNAs) and for protein-coding genes.<br><br> <br><br>Figure S1. Cell morphology in RPMI medium at 30°C<br><br>The wild-type strain (CW1757), cup9Δ/Δ mutant (YI192), efg1Δ/Δ mutant (CW1792), cup9Δ/Δ efg1Δ/Δ mutant (CW1796), cup9Δ/Δ efg1(Δ/Δ)+/+ reconstituted strain (CW1785), and efg1(Δ/Δ)+/+ reconstituted strain (CW1779) were incubated in RPMI at 30°C for 6 h. Images were taken by Zeiss Axio Observer Z.1 fluorescence microscope and a 20x objective. The scale bar corresponds to 10 microns.<br><br> <br><br>Figure S2. Caspofungin sensitivity of cell wall integrity mutants in RPMI medium.<br><br>Wild type strain CW696 and previously described caspofungin hypersensitive mutants (Bruno et al. 2006; Rauceo et al. 2008; Blankenship et al. 2010) were serially diluted onto RPMI solid medium with or without caspofungin. Cells were incubated for 3 days at 30°C. <br><br><br><br>Figure S3. Caspofungin sensitivity at 37°C<br><br>The wild-type (CW542), cup9Δ/Δ mutant (YI192), and CUP9-complemented (YI243) strains were incubated on RPMI medium at 37°C with or without caspofungin, as indicated in the figure. Spotting assays were photographed after 2 or 3 days.<br><br>