CUT&Tag of H3K27me3, E9.5 Neural Tube defects mouse brain

To study the histone H3K27me3 enrichment in E9.5d mouse brains with neural tube defects or control, we performed H3K27m3 in mouse brains.NTDs mouse model: CD-1 mice (7–8 weeks old) were provided by Beijing Vital River laboratory Animal Technology Co., Ltd, China (no: 110011200105514958, 16-25g). Female CD-1 mice were fed on low folate diet for more than 8 weeks. Sexually matured individuals were mated overnight; vaginal plug was detected at 8:00 am in the following morning and was designated as E0.5 day. NTDs mouse models were induced by intraperitoneal injection with 1.5 mg/kg of MTX (Sigma-Aldrich, USA) on E7.5. On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope. All samples with NTDs clinical manifestations were collected for experiments. Overall design: We compared the h3K27me3 of control and neural tube defects mouse brains of E9.5d. Three biological replicates are set for each group, con1, con2, con3 were control groups from control. And M1, M2 and M3 were from NTDs groups.