Raw diffraction images of YidC (phasing data by Hg-MAD)

Diffraction images of mercury-bound Bacillus halodurans YidC mutants for Hg-MAD phasing. This phase information was used for PDB entries 3WO6 and 3WO7 (Kumazaki et al. 2014). All data were collected from loop-harvested crystals on BL32XU, SPring-8 at wavelengths of 1.00000 Å (peak) or 1.00945 Å (edge) using the MX225HE CCD detector. From the M146C mutant, helical (360 or 720°/crystal, 2°/frame) datasets were collected using 10×1 μm2 beam. From a Y150C mutant, a helical (360°/crystal, 2.5°/frame) dataset was collected using 15×1 μm2 beam, which was used for phase determination. The crystals belonged to space group P21 with unit cell parameter a~44, b~60, c~60 Å, β~100°.