An integrated single-cell RNA-seq atlas of the mouse hypothalamic paraventricular nucleus links transcriptional and function types

The hypothalamic paraventricular nucleus (PVN) is a highly complex brain region that is crucial for homeostatic
regulation through neuroendocrine signalling, outflow of the autonomic nervous system (ANS), and projections
to other brain areas. The past years, single-cell datasets of the hypothalamus have contributed immensely
to the current understanding of the diverse hypothalamic cellular composition. While the PVN has been
adequately classified functionally, its molecular classification is currently still insufficient.

To address this, we created a detailed atlas of PVN transcriptional cell types by integrating various PVN
single-cell datasets into a recently published hypothalamus single-cell transcriptome atlas. Furthermore, we
functionally profiled transcriptional cell types, based on relevant literature, existing retrograde tracing data
and existing single-cell data of a PVN-projection target region.