Gene expression profile of mouse liver in response to activation of PPARA by WY-14643.

Gene expression in adult male mouse liver was assayed by RNA-seq, as part of a study of liver transcriptomic changes in response to activation of PPARA by WY-14643. Overall design: Twelve independent pools, comprised of RNA isolated from n=3-5 individual 12 wk old male livers per pool, were prepared and used for RNA-seq. Three pools were untreated, intact male mice livers; three pools were treated (WY-14,643), intact male mice livers; three pools were untreated, PPARA-KO mouse livers and three pools were treated, PPARA-KO mice livers. Wy-14,643 treatment was via the diet, at 0.1% for 48 hr.