FHL3 promotes the formation of fast glycolytic muscle fibers by interacting with YY1 and muscle glycolytic metabolism

Composition of distinct muscle fiber types plays an important role in the regulation of skeletal muscle metabolism, as well as animal meat production.Four and a half LIM domain protein 3 (FHL3) is highly expressed in fast glycolytic muscle fibers and differentially regulates the expression of MyHC isoforms at cellular levels.Here we knocked out FHL3 in C2C12 cells by CRISPR/Cas9 technology.RNA sequencing analyses revealed that the expression of MYH7 in KO cells was significantly increased, while the expression of MYH1,MYH2 and MYH4 was significantly decreased.Further comfirm FHL3 regulates the transformation of muscle fiber types. Comparative gene expression profiling analysis of RNA-seq data for WT and FHL3 KO C2C12 cells which were differentiated for 4 days.