Sensitivity as well as intra-assay and inter-assay reproducibility of the 11 reverse transcriptase enzymes assessed in this study.

sd = standard deviation, cv = coefficient of variation. A) Sensitivity and reproducibility (mean Cq±sd, n = 2) within a run; B) Intra-assay variability [% cv = (sd/mean Cq)x100] based on replicate runs; C) Inter-run variability of cDNA quantification derived from 1,000 copies of Armored HIV RNA with ACC reverse transcriptase providing the calibration curve. NA denotes failed amplifications or values excluded due to non-specific products, while Cq values with no sd amplified only once.