HspB2 is highly specific for its targets as revealed by a comparison of HspB2 and HspB5 binding partners.

Twelve binding partners from the HspB2 Y2H screen were tested for their ability to bind HspB2 or HspB5. The columns are as follows: gene (the name of the gene encoded on the library ‘prey’ plasmid retrieved from the Y2H screen), plasmid (the pJG number of the purified plasmid), HspB2 (the Y2H protein-protein interaction result using HspB2 as bait), HspB5 (the Y2H protein-protein interaction result using HspB5 as bait), and EV (the Y2H result using an empty vector control). Strong, medium or weak indicates apparent strength of the interaction as determined by growth on selective media (SD–Trp–Leu–His–Ade); NG means no detectable growth. HspB5 was used as a positive control and was also retrieved from the yeast two-hybrid screen.