Impact of vitamin C supplementation on lipid peroxidation and reduced glutathione levels in the liver tissue of mice with cyclophosphamide-induced immunosuppression

ABSTRACT Objective: To investigate the effects of vitamin C supplementation on the levels of lipid peroxidation and reduced glutathione in the liver tissue of mice immunosuppressed with cyclophosphamide. Methods: Thirty-two 45-day-old female Swiss mice were divided into four groups of eight animals each as follows: control (distilled water); vitamin C (50 mg/kg); cyclophosphamide (100 + 150 mg/kg); and treatment (vitamin C 50 mg/kg + cyclophosphamide 100 +150 mg/kg). The substances were provided intraperitoneally for six days, and on the seventh day, the mice were euthanized. The biochemical analyses of lipid peroxidation (quantification of thiobarbituric acid-reactive substances) and reduced glutathione (estimate of non-protein thiols) were performed on liver tissue. Results: Cyclophosphamide increased the levels of lipid peroxidation (p