Table 1 in Bringing order to a complex system: phenotypic and genotypic evidence contribute to the taxonomy of Tityus (Scorpiones, Buthidae) and support the description of a new species

Table 1. List of primers used to amplify DNA sequences of Tityus species. Abbreviations: F forward R reverse T temperature.
LocusPrimerSequencesDirectionAnnealing (T, °C)ReferenceCOILCO1490-jj25'- CHA CWA AYC AYA ARG AYA TYG GF49.3-62.0Astrin et al. (2016)COIHCO2198-jj25'- ANA CTT CNG GRT GNC CAA ARA ATC AR57.9-66.7Astrin et al. (2016)12S12Sai5'- AAA CTA GGA TTA GAT ACC CTA TTA TF52.3Kocher et al. (1989)12S12Sbi5'- AAG AGC GAC GGG CGA TGT GTR64.6Kocher et al. (1989)12S12Sop2r5' CCC TTA AAY YTA CTT TGT TAC GAC CR50Pinto-da-Rocha et al. (2014)16S16Sbr5'- CTC CGG TTT GAA CTC AGA TCAF57.7Simon et al. (1994)16S16S_F5'- CGA TTT GAA CTC AGA TCAF49.3Gantenbein et al. (1999)16S16Sbr_mod5'- GTG CAA AGG TAG CAT AAT CAR53.7Gantenbein et al. (1999)28S28Sa (Sad3)5'- GAC CCG TCT TGA AAC ACG GAF60.3Whiting et al. (1997)28S28Srd5b5'- CCA CAG CGC CAG TTC TGC TTA CR64.2Schwendinger and Giribet (2005)28S28SBout5'- CCC ACA GCG CCA GTT CTG CTT ACCR68Schulmeister (2003)