Molecular Pathways are influenced by Progranulin in a neuronal cell model

Gene expression microarrays were used to identify how the mRNA phenotype of NSC-34 cells responds to PGRN expression levels. PGRN expression correlated well with molecular phenotypes that included mRNA pathways associated with the regulation of the cytoskeleton Gene expression profile was determined using Illumina Mouse Whole-Genome Expression BeadChips (MouseWG-6 v2.0 Total RNA obtained from NSC-34 vector control cells, and cells that either stably over-express human PGRN (NSC-34/hPGRN) or under-express murine PGRN (NSC-34/shPGRN) were compared using Illumina Mouse Whole--Genome Expression BeadChips (MouseWG-6 v2).