Single cell RNA-seq of forebrain cells isolated from wild-type mouse, rat, and chimeric mouse

We performed single cell RNA-seq to quantify gene expression in forebrain tissue from the wild-type mouse, rat, and chimeric mouse. Single cell data from WT mouse or rat was used to perform integrative analysis for identifying rat cells and mouse cells in the chimaeras, respectively. Forebrain tissues from CCBC-Hesx1+rESCs mice (n = 3 mice) , WT rat (n =1 rat) and WT mouse (n =1 mouse) at P0 were subjected to scRNA-seq.