Supporting Information S1 - Seamless Gene Tagging by Endonuclease-Driven Homologous Recombination

Supporting Information S1 contains one supplementary protocol (Protocol S1), three supplementary figures (Figures S1–S3) and five supplementary tables (Tables S1–S5). Protocol S1: High fidelity PCR amplification of tagging modules. Figure S1: Methods for chromosomal gene tagging in S. cerevisiae. Figure S2: Alternative routes of DSB repair upon marker excision in the HXT6 locus. Figure S3: High-throughput strain construction by seamless tagging in combination with SGA technology. Table S1: Plasmids. Table S2: Yeast strains. Table S3. Oligonucleotides. Table S4: ORFs with potential for ectopic module excision during seamless N-terminal tagging. Table S5: ORFs with potential for ectopic module excision during seamless C-terminal tagging.
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