Gene expression changes in IDH mutant or IDH wildtype gliomas treated with All-trans Retinoic Acid

IDH mutant cells are deficient in retinoic acid production, which impacts downstream gene expression and pathways. We looked at the effect of supplementing IDH mutant glioma cells with ATRA on gene expression and downstream pathways. We compared gene expression changes with IDH WT cells that were also treated with ATRA Primary IDH mutant/ wildtype glioma cells were cultured in vitro and treated with 1uM ATRA for 48 hours. DMSO treated cells were used as controls. After treatment, RNA was extracted from cells and sent for microarray analysis at the Genomics core at the University of Pittsburgh