Data for: Dysregulation of mTOR signaling mediates common neurite and migration defects in both idiopathic and 16p11.2 deletion autism neural precursor cells

Autism spectrum disorder (ASD) is defined by common behavioral characteristics, raising the possibility of shared pathogenic mechanisms. Yet, vast clinical and etiological heterogeneity suggests personalized phenotypes. Surprisingly, our iPSC studies find that six individuals from two distinct ASD subtypes, idiopathic and 16p11.2 deletion, have common reductions in neural precursor cell (NPC) neurite outgrowth and migration even though whole genome sequencing demonstrates no genetic overlap between the datasets. To identify signaling differences that may contribute to these developmental defects, an unbiased phospho-(p)-proteome screen was performed. Surprisingly, despite the genetic heterogeneity, hundreds of shared p-peptides were identified between autism subtypes including the mTOR pathway. mTOR signaling alterations were confirmed in all NPCs across both ASD subtypes and mTOR modulation rescued ASD phenotypes and reproduced autism NPC-associated phenotypes in control NPCs. Thus, ou..., The entire dataset represents source data for the Figures presented in our manuscript published in the journal Elife \" Dysregulation of mTOR signaling mediates common neurite and migration defects in both idiopathic and 16p11.2 deletion autism neural precursor cells”. The source data is a requirement for publication in Elife and will aid others who would like to replicate the Figures and potentially utilize the data in their future assessments. With this in mind, much of the methods utilized in collecting our data and the nomenclature of the files in this data bank can largely be contextualized by referencing our manuscript. For example, Figure1B_SourceData is the data utilized to create Figure 1B in the above manuscript.
The manuscript focused on cell culture data from a bench lab setting. Specifically, this work focused on cells known as neural precursor cells (NPCs), which are early brain cells that ultimately go on to develop into neurons, astrocytes, and oligodendrocytes (the matur..., , # Data for: Dysregulation of mTOR signaling mediates common neurite and migration defects in both idiopathic and 16p11.2 deletion autism neural precursor cells

[https://doi.org/10.5061/dryad.6wwpzgn5v](https://doi.org/10.5061/dryad.6wwpzgn5v)

As described in the methods section, the entire dataset represents source data for the Figures presented in our manuscript published in the journal Elife \" Dysregulation of mTOR signaling mediates common neurite and migration defects in both idiopathic and 16p11.2 deletion autism neural precursor cells”. The source data is a requirement for publication in Elife and will aid others who would like to replicate the published figures and potentially utilize the data in their future assessments. With this in mind, much of the methods utilized in collecting our data and the nomenclature of the files in this data bank can largely be contextualized by referencing our manuscript. For example, Figure1B_SourceData is the data utilized to create Figure 1B in...