The use of nanopore sequencing to analyze the chloroplast transcriptome part 2 – bioinformatic analyzes and virtual RN blots

Nanopore sequencing of full-length cDNAs offers unprecedented details of the plastid RNA metabolism. After the generation of the nanopore reads, several bioinformatic steps are required to analyze the

DataCite Commons2025-04-16 更新60

Transcriptome sequencing using nanopore sequencing

For general RNA-Seq using short read sequencer, it is difficult to reveal entire structure of transcripts. To overcome this problem, we performed the MinION sequencing of full-length cDNA synthesized

NIAID Data Ecosystem50

Transcriptome assembly for nanopore sequencing

We evaluated the performance of multiple leading transcriptome assembly algorithms on their ability to accurately reconstruct RNA transcript isoforms. We specifically focused on deep nanopore sequenci

NIAID Data Ecosystem10

The use of nanopore sequencing to analyze the chloroplast transcriptome part ...