Transcriptomic profiling investigating altered cellular pathways upon QKI depletion in mouse eye lens tissue, mouse neural stem cell-derived lens progenitor-like cells (NLPCs), and human lens epithelial cell line (HLE-B3).

Significantly altered cellular pathways in the transcriptomic profiling upon QKI depletion potentially play an important role in protein homeostasis in eye lens cells. Mouse eye lens tissues were isolated from the mice with tamoxifen-induced Qk deletion under the control of Nestin promoter and the control mice. NLPCs were differentiated from the neural stem cells isolated from Nestin-CreERT2; QkL/L, and 4OHT treatment induced the Qk deletion. HLE-B3 cells were obtained from ATCC (CRL-11421), and QKI KO was conducted using CRISPR-Cas9 system.